Thermo Scientific PikoReal 24 Real-Time PCR System

Description

The Thermo Scientific PikoReal 24 cycler is ideal for starting real-time PCR for low sample throughput (24-well format). The PikoReal software offers simple and intuitive cycler operation and allows easy online control from your computer. The cycler has 5 detection channels, which detect hydrolysis (5’Nuclease / TaqMan) probes either as single color or multiplex samples. Thermal block Block format: 24 well Sample volume: 10 – 50 μl Consumables: Low profile strip tubes and PCR tubes Max heating rate: > 5°C/sec Max cooling rate: 4.5°C/sec Temperature range: 4-99.9°C Temperature accuracy: +/-0.2°C Temperature uniformity: ±0.3°C at 95°C, ±0.15°C at 60°C, ±0.2°C at 72°C Heated Lid Temperature range: 50-110°C Control: Automatic temperature and pressing setting Optics Excitation: 5 LEDs Excitation range: 475-640 nm Detection: CCD Detection channels Channel 1 – Excitation: 475-500nm, Emission: 520-550nm, Pre-calibrated dyes: FAM Channel 2 – Excitation: 515-535nm, Emission: 557-590nm, Pre-calibrated dyes: HEX, Yakima Yellow Channel 3 – Excitation: 570-590nm, Emission: 615-650nm, Pre-calibrated dyes: ROX, Texas Red Channel 4 – Excitation: 600-640nm, Emission: 666-740nm, Pre-calibrated dyes: Cy 5 Channel 5 – Excitation: 475-500nm, Emission: 520-590nm, Pre-calibrated dyes: SYBR Green Multiplex: Up to 4 targets Dynamic range: 10 logs Sensitivity: 1 copy Analysis modes Absolute quantification; Relative quantification; Melt curve analysis; Allelic discrimination; High Resolution Melt (HRM) Stand-alone or PC control: The PikoReal qPCR system is controlled by PikoReal software, which allows remote monitoring of any qPCR experiment online from your own computer. Alternatively, you can save the qPCR protocol on a USB stick and load to the PikoReal instrument for stand-alone use. PikoReal software include modules for absolute and relative quantification, melting curve analysis, allelic discrimination and High Resolution Melting (HRM). Advanced algorithms provide effortless data normalization without the need for an external reference dye. Including software