BioFire FilmArray PCR System (Idaho Technology)

Description

The FilmArray pouch is a multiplex PCR assay that stores all the necessary reagents for sample preparation, reverse transcription PCR, PCR, and detection in a freeze-dried format. During a test run, the system extracts and purifies all nucleic acids from the unprocessed sample. Next, it performs nested multiplex PCR in two stages. The first stage includes a single, large volume, multiplexed reaction. The second stage includes individual, single-plex reactions to detect the products from the first stage. Using endpoint melting curve data, the system software automatically analyzes the results for each target on the panel. When the run is complete, the software reports whether each pathogen is detected in the sample. This information is printed in an automated response at the end of the test run.