ProteinSimple Wes Automated Western Blot System (Bio-Techne)

Description

A Simple Western size assay is an automated Western – no gels, no transfer devices, no blots, no film and no manual analysis. Simple Westerns take place in a capillary. Wes automates all steps of the process for you including sample loading, protein separation, immunoprobing or labeling, washing, detection and data analysis. Up to 25 samples can be processed in a single run. Wes performs Simple Western size-based assays: Immunoassay and Total Protein assay. All you have to do is pipette your samples and immunoreagents into Wes’s pre-filled plate, load the capillary cartridge and press start. Sample Required Total Protein: 0.3-1.2 µg lysate Sample Required Immunoassay Charge: 0.6-1.2 µg Volume Required Total Protein: 3 µL/well Volume Required Immunoassay Charge: 3 µL/well Size or pI Range Total Protein: Molecular weight (MW) ladders range from 2-440 kDa Size or pI Range Immunoassay Charge: Molecular weight (MW) ladders range from 2-440 kDa Sizing CV Total Protein: <10% Sizing CV Immunoassay Charge: <10% Intra-assay CV Total Protein: <15% Intra-assay CV Immunoassay Charge: <15% Resolution (± percent difference in MW) Total Protein: ± 15-20% for MW <20 kDa, ±10 % for MW > 20 kDa Resolution (± percent difference in MW) Immunoassay Charge: ± 15-20% for MW <20 kDa, ±10 % for MW > 20 kDa Quantitation CV Total Protein: <20% Quantitation CV Immunoassay Charge: <20% Dynamic Range Total Protein: 2-3 logs Dynamic Range Immunoassay Charge: 3 logs Sensitivity Total Protein: ng Sensitivity Immunoassay Charge: Low pg Capillary: 5 cm, 100 µm, 400 nL Dimensions (HxWxD): 360 x 360 x 570 mm Weight: 23 kg