Cytiva Biacore T200 SPR Biosensor Analyser

Specifications

Condition

Good

Manufacturer

Cytiva

Model

Biacore T200

KD

Weak interactions with KD in the mM range

Ka

Proteins: 10^3 to 3 × 10^9 M-1s-1; LMW molecules: 10^3 to 5 × 10^7 M-1s-1

Kd

10^-5 to 1 s-1

Power

Processing Unit: Autorange 100 to 240 VAC, 50 to 60 Hz, Class 1 equipment. Power consumption: Processing Unit: 4.0 A

Recovery

1.5 µL analyte recovery volume

Detection

Surface Plasmon Resonancebiosensor

Automation

48 h unattended operation

Flow Cells

Four

Mw Detection

No lower limit for organic molecules

Baseline Drift

Typically < 0.3 RU/min

Baseline Noise

Typically < 0.03 RU

Buffer Selector

Automatic switching between four buffers

Flow Rate Range

From 1 to 100 µL/min

Flow Cell Height

40 µm

Flow Cell Volume

0.06 µL

Injection Volume

2 to 350 µL

Analysis Temperature

4°C to 45°C

Instrument Weight Kg

60

Operating Conditions

Operation 18°C to 33°C; relative humidity 20% to 80%, non-condensing; altitude up to 2000 m. Bench area 65 x 65 cm plus approx. 60 x 60 cm for the computer; at least 20 cm clearance on all sides.

Sample Concentration

> 1 pM

Instrument Dimensions

[object Object]

Required Sample Volume

Injection volume plus 20 to 50 µL

Software Compatibility

Biacore T200 Control and Evaluation Software; result files also open in Biacore Insight Evaluation Software. Optional Biacore T200 GxP Package. OS: Windows 10 Professional or Enterprise, 64-bit. Minimum PC: 3.0 GHz processor, RAM > 1 GB free, HDD > 2 GB free, graphics at least 1280 × 1024, 2 RS-232 ports.

Sample Refractive Index

1.33 to 1.40

Sample Storage Temperature

4°C to 45°C