Invitrogen MPK5000 Neon Transfection System

Description

The Neon Transfection System enables fast and efficient delivery of nucleic acids into all mammalian cell types, including primary, stem, and difficult-to-transfect cells. The flexible and open system allows the user to perform high-quality transfections using optimized or user-defined protocols in three simple steps with as few as 2 x 104 cells per reaction. A novel reaction chamber provides a dramatic increase in transfection efficiency and cell viability. The Neon Transfection System is: Efficient – up to 90% in many cell types, including difficult-to-transfect cells, primary, and stem cells Flexible – easily transfect from 2 x 104 cells to 6 x 106 cells per reaction Simple – easy to use, with a single reagent kit for all cell types Versatile – open system allows electroporation parameters to be optimized freely Specifications Format: Electroporation Pipette Tip System: Neon Transfection System Cell Type: Established Cell Lines, Hard-to-Transfect Cells, Stem Cells, Primary Cells Power Limit: 300 W Current Limit: 3.0 – 6.0 A Reaction Time: 1 sec Voltage Limit: 0.5 – 2.5 kV Classification: Animal Origin-Free, Reduced Serum, Chemically Defined, Low-Protein, Reduced Growth Factors, Xeno-Free Serum Compatible: Yes Compatible Products: Transfection Grade Nucleic Acid purification kits, siRNA vectors, DNA vectors Sample Loading Volume: Depends on kit used Sample Type (Specific): Plasmid DNA, Synthetic siRNA Purity or Quality Grade: Transfection Grade Starting Material (Cell #): 2 x 104 – 6 x 106 cells; Varies by cell type