Thermo Scientific Ion Torrent Personal Genome Machine (PGM) System

Description

The Ion Personal Genome Machine (PGM) System combines semiconductor sequencing technology with natural biochemistry to directly translate chemical information into digital data, democratizing sequencing and making it accessible to your lab. This established system leverages the exponential improvements in the semiconductor industry (known as Moore’s Law) to provide scalability and flexibility for various applications. The Ion PGM System uses natural sequencing chemistry, which eliminates the need for expensive optics and reduces complex chemistries to measure natural DNA extension. The system leverages direct, real-time sequencing detection, providing sequencing results typically in 3–7 hours. CPU: Dual 6-core 2.66 GHz CPUs Capacity: 1 chip per run Chip Compatibility: Ion 314 Chip v2 BC, Ion 316 Chip v2 BC, Ion 318 Chip v2 BC Current Limit: 6.7 A (max) Data Output: BAM Files, FASTQ Files, SFF Files, VCF Files Display Interface: Touchscreen User Interface External Computer: Torrent Server High Throughput Compatibility: High Throughput-Compatible, Multiplexing Instrument Memory: 48 GB RAM, Eight 2 TB Hard drives in RAID 5 Operating System: Ubuntu 10.04 Output: Ion 314 Chip v2: Up to 100 Mb, Ion 316 Chip v2: Up to 1 Gb, Ion 318 Chip v2: Up to 2 Gb Read Length: 35–400 bases (average: 200 bases) Reads⁄Run: Ion 314 Chip v2: 400-500 thousand, Ion 316 Chip v2: 2-3 million, Ion 318 Chip v2: 4-5.5 million Run Time: Ion 314 Chip v2: 2.3 to 3.7 hr, Ion 316 Chip v2: 3.0 to 4.9 hr, Ion 318 Chip v2: 4.4 to 7.3 hr Sequencing Scale: 3 Mb to 2 Gb Technique: Post-Light Ion Semiconductor Sequencing Throughput: Up to 2 Gb per run Voltage Limit: 100 V (min) to 240 V (max) Dimensions (H x W x D): 530 x 240 x 200 mm Weight: 30 kg The Ion OneTouch 2 System performs template amplification and enrichment as part of the manual workflow for the Ion PGM, Ion Proton, and Ion S5 and Ion S5 XL systems. It provides scalable template preparation for all Ion semiconductor chips. With its small footprint, the system fits easily on any bench top. The Ion OneTouch 2 System integrates multiple manual template preparation steps into a single system. This enables parallel processing of multiple samples per day through modular design. It is flexible and supports scalable template preparation for all Ion semiconductor sequencing chips with up to 400 bases on the Ion 520/530 chips, up to 200 bases on the Ion 540 chip, up to 400 bases on the Ion PGM System, and up to 200 bases on the Ion Proton System. The need for gas is eliminated by the use of a peristaltic pump. Chip Compatibility: Ion 314 Chip, Ion 316 Chip, Ion 318 Chip, Ion PI Chip High Throughput Compatibility: Automated Protocols, High Throughput-Compatible, Multiplexing Library Type: Fragment Library, Mate-Paired Library, Targeted Sequencing Library, cDNA Library Multiplex Capability: Up to 384 barcoded libraries Run Time: Hands-on time: Minutes, Ion PGM System: 5 hours total for template preparation