Illumina HiSeq 2500 Sequencing System

Description

Illumina HiSeq 2500 Sequencing System with cBot Flow Cell Prep System HiSeq 2500 System is a powerhouse of Illumina sequencers for high throughput applications such as small and large whole genome sequencing, exome sequencing, targeted sequencing, whole transcriptome, mRNA-seq, and shotgun metagenomics. It has the capacity to produce 160 Gb of data per day (or 1 Tb per run) and the system can be configured to run 1 or 2 flow cells simultaneously. In high output mode, it can process up to 8 human genomes at 30x coverage with one genome being sequenced in 27 hours. HiSeq 2500 also features a touch screen control interface and a software with guided step-by-step workflows. Quality statistics can be monitored in real-time from the intsrument, on a remote computer, or even on an internet browser. The Illumina sequencing systems utilize a well-established sequencing by synthesis (SBS) method in which fluorescently labeled nucleotide bases are detected as they are incorporated into DNA template strands. All four reversible terminator-bound dNTPs are present in each sequencing cycle; this feature minimizes bias of incorporation and greatly reduces raw error rates.